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custom designed brca1 adenovirus  (Vector Biolabs)


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    Vector Biolabs custom designed brca1 adenovirus
    FIGURE 1. <t>BRCA1</t> is constitutively expressed in aortic SMCs. A, Representative agarose gel image and (B) immunoblot confirming the constitutive presenceofBRCA1transcriptandproteininC57Bl/6JMASMCs, WKY RASMCs and HASMCs. n ¼ 5 per group. SMC, Smooth muscle cells; BRCA1, breast cancer 1, early onset gene; MW, molecular weight marker; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.
    Custom Designed Brca1 Adenovirus, supplied by Vector Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/custom designed brca1 adenovirus/product/Vector Biolabs
    Average 96 stars, based on 4 article reviews
    custom designed brca1 adenovirus - by Bioz Stars, 2026-05
    96/100 stars

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    1) Product Images from "BRCA1 shields vascular smooth muscle cells from oxidative stress."

    Article Title: BRCA1 shields vascular smooth muscle cells from oxidative stress.

    Journal: The Journal of thoracic and cardiovascular surgery

    doi: 10.1016/j.jtcvs.2013.09.060

    FIGURE 1. BRCA1 is constitutively expressed in aortic SMCs. A, Representative agarose gel image and (B) immunoblot confirming the constitutive presenceofBRCA1transcriptandproteininC57Bl/6JMASMCs, WKY RASMCs and HASMCs. n ¼ 5 per group. SMC, Smooth muscle cells; BRCA1, breast cancer 1, early onset gene; MW, molecular weight marker; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.
    Figure Legend Snippet: FIGURE 1. BRCA1 is constitutively expressed in aortic SMCs. A, Representative agarose gel image and (B) immunoblot confirming the constitutive presenceofBRCA1transcriptandproteininC57Bl/6JMASMCs, WKY RASMCs and HASMCs. n ¼ 5 per group. SMC, Smooth muscle cells; BRCA1, breast cancer 1, early onset gene; MW, molecular weight marker; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.

    Techniques Used: Agarose Gel Electrophoresis, Western Blot, Molecular Weight, Marker

    FIGURE 2. BRCA1 overexpression reduces oxidative stress in HASMCs via the NADPH oxidase system. ROS production was determined by (A) CM-DCF and (B) DHE fluorescence in HASMCs stressed for 24 hours with H2O2. Representative Western blots and semiquantitative data for the NADPH oxidase subunits (C) Nox1 and (D). p47phox in naive and adenovirus-treated HASMCs. GAPDH acted as the housekeeping protein. n ¼ 5 per group. *P <.05 versus the corresponding untreated group; yP <.05 versus the H2O2-treated Ad-null–transfected group. CM-DCF, 6-Chloromethyl- 20,70-dichlorodihydrofluorescein diacetate, acetyl ester; Ad-null, control adenovirus; Ad-BRCA1, BRCA1 adenovirus; DHE, dihydroethidium; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.
    Figure Legend Snippet: FIGURE 2. BRCA1 overexpression reduces oxidative stress in HASMCs via the NADPH oxidase system. ROS production was determined by (A) CM-DCF and (B) DHE fluorescence in HASMCs stressed for 24 hours with H2O2. Representative Western blots and semiquantitative data for the NADPH oxidase subunits (C) Nox1 and (D). p47phox in naive and adenovirus-treated HASMCs. GAPDH acted as the housekeeping protein. n ¼ 5 per group. *P <.05 versus the corresponding untreated group; yP <.05 versus the H2O2-treated Ad-null–transfected group. CM-DCF, 6-Chloromethyl- 20,70-dichlorodihydrofluorescein diacetate, acetyl ester; Ad-null, control adenovirus; Ad-BRCA1, BRCA1 adenovirus; DHE, dihydroethidium; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.

    Techniques Used: Over Expression, Western Blot, Transfection, Control

    FIGURE 3. Aortas and RASMCs from SHR rats express less BRCA1 than those from WKY rats. BRCA1 transcripts in (A) WKY and SHR rat aorta and (B) RASMC homogenates were assessed via qPCR. n ¼ 5 per group. *P<.05 versus WKY group. BRCA1, Breast cancer 1, early onset gene; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; WKY, Wistar- Kyoto rat; SHR, spontaneously hypertensive rat; RASMC, rat aortic smooth muscle cells.
    Figure Legend Snippet: FIGURE 3. Aortas and RASMCs from SHR rats express less BRCA1 than those from WKY rats. BRCA1 transcripts in (A) WKY and SHR rat aorta and (B) RASMC homogenates were assessed via qPCR. n ¼ 5 per group. *P<.05 versus WKY group. BRCA1, Breast cancer 1, early onset gene; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; WKY, Wistar- Kyoto rat; SHR, spontaneously hypertensive rat; RASMC, rat aortic smooth muscle cells.

    Techniques Used:

    FIGURE 4. BRCA1 overexpression reduces oxidative stress in SHR RASMCs via the NADPH oxidase system. A, ROS production and (B) NADPH oxidase activity were assessed by CM-DCF fluorescence and lucigenin-enhanced chemiluminescence, respectively, in homogenates of WKY RASMCs and adenovirus- transfectedSHRRASMCs(n¼ 6pergroup).Representative WesternblotsandsemiquantitativedatafortheNADPHoxidasesubunits(C)Nox1and(D)p47phox in WKYand SHR RASMC homogenates. n ¼ 5 per group. *P<.05 versus WKY group; yP<.05 versus Ad-null SHR group. CM-DCF, 6-Chloromethyl-20,70- dichlorodihydrofluorescein diacetate, acetyl ester; WKY, Wistar-Kyoto rat; Ad-null, control adenovirus; Ad-BRCA1, BRCA1 adenovirus; SHR, spontaneously hypertensive rat; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; NADPH, nicotinamide adenine dinucleotide phosphate.
    Figure Legend Snippet: FIGURE 4. BRCA1 overexpression reduces oxidative stress in SHR RASMCs via the NADPH oxidase system. A, ROS production and (B) NADPH oxidase activity were assessed by CM-DCF fluorescence and lucigenin-enhanced chemiluminescence, respectively, in homogenates of WKY RASMCs and adenovirus- transfectedSHRRASMCs(n¼ 6pergroup).Representative WesternblotsandsemiquantitativedatafortheNADPHoxidasesubunits(C)Nox1and(D)p47phox in WKYand SHR RASMC homogenates. n ¼ 5 per group. *P<.05 versus WKY group; yP<.05 versus Ad-null SHR group. CM-DCF, 6-Chloromethyl-20,70- dichlorodihydrofluorescein diacetate, acetyl ester; WKY, Wistar-Kyoto rat; Ad-null, control adenovirus; Ad-BRCA1, BRCA1 adenovirus; SHR, spontaneously hypertensive rat; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; NADPH, nicotinamide adenine dinucleotide phosphate.

    Techniques Used: Over Expression, Activity Assay, Control

    FIGURE 5. BRCA1 overexpression lowers systolic and diastolic blood pressure in SHR rats. A, Blood pressure and (B) heart rates were continuously recorded via radiotelemetry in SHR rats treated with either Ad-null or Ad-BRCA1 (5 3 1010 IU/rat, intravenously). n ¼ 9 per group. *P < .05 versus Ad-null. Ad-null, Control adenovirus; Ad-BRCA1, BRCA1 adenovirus.
    Figure Legend Snippet: FIGURE 5. BRCA1 overexpression lowers systolic and diastolic blood pressure in SHR rats. A, Blood pressure and (B) heart rates were continuously recorded via radiotelemetry in SHR rats treated with either Ad-null or Ad-BRCA1 (5 3 1010 IU/rat, intravenously). n ¼ 9 per group. *P < .05 versus Ad-null. Ad-null, Control adenovirus; Ad-BRCA1, BRCA1 adenovirus.

    Techniques Used: Over Expression, Control

    FIGURE 6. BRCA1 overexpression reduces aortic oxidative stress in SHR rats via the NADPH oxidase system. A, ROS production (n ¼ 10 per group) and (B) NADPH oxidase activity (n ¼ 6 pergroup) in aorta homogenatesfrom WKYand adenovirus-treated SHR rats were assessed by lucigenin-enhanced chem- iluminescence. C, Representative Western blots and semiquantitative data for the NADPH oxidase subunits Nox1 and p47phox in WKY and adenovirus- treated SHR rat aortas. GAPDH acted as the housekeeping protein. n ¼ 6 per group. *P<.05 versus WKY group; yP<.05 versus Ad-null SHR group. ROS, Reactive oxygen species; WKY, Wistar-Kyoto rat; Ad-null, control adenovirus; Ad-BRCA1, BRCA1 adenovirus; SHR, spontaneously hypertensive rat; RLU, relative light units; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; NADPH, nicotinamide adenine dinucleotide phosphate.
    Figure Legend Snippet: FIGURE 6. BRCA1 overexpression reduces aortic oxidative stress in SHR rats via the NADPH oxidase system. A, ROS production (n ¼ 10 per group) and (B) NADPH oxidase activity (n ¼ 6 pergroup) in aorta homogenatesfrom WKYand adenovirus-treated SHR rats were assessed by lucigenin-enhanced chem- iluminescence. C, Representative Western blots and semiquantitative data for the NADPH oxidase subunits Nox1 and p47phox in WKY and adenovirus- treated SHR rat aortas. GAPDH acted as the housekeeping protein. n ¼ 6 per group. *P<.05 versus WKY group; yP<.05 versus Ad-null SHR group. ROS, Reactive oxygen species; WKY, Wistar-Kyoto rat; Ad-null, control adenovirus; Ad-BRCA1, BRCA1 adenovirus; SHR, spontaneously hypertensive rat; RLU, relative light units; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; NADPH, nicotinamide adenine dinucleotide phosphate.

    Techniques Used: Over Expression, Activity Assay, Western Blot, Control

    FIGURE 7. Aortas from Ad-BRCA1–treated SHR rats exhibit less H2A.X phosphorylation than those from Ad-null–treated SHR rats. gH2A.X levels were assessed via immunohistochemistry. Images are representative of n ¼ 5 per group. *P <.05 versus WKY group; yP <.05 versus Ad-null SHR group. WKY, Wistar-Kyoto rat; SHR, spontaneously hypertensive rat; Ad-null, control adenovirus; Ad-BRCA1, BRCA1 adenovirus.
    Figure Legend Snippet: FIGURE 7. Aortas from Ad-BRCA1–treated SHR rats exhibit less H2A.X phosphorylation than those from Ad-null–treated SHR rats. gH2A.X levels were assessed via immunohistochemistry. Images are representative of n ¼ 5 per group. *P <.05 versus WKY group; yP <.05 versus Ad-null SHR group. WKY, Wistar-Kyoto rat; SHR, spontaneously hypertensive rat; Ad-null, control adenovirus; Ad-BRCA1, BRCA1 adenovirus.

    Techniques Used: Phospho-proteomics, Immunohistochemistry, Control

    FIGURE 8. Aortas from Ad-BRCA1–treated SHR rats exhibit more RAD51 foci formation than those from Ad-null–treated SHR rats. RAD51 foci were quantified via immunohistochemistry. Images are representative of n ¼ 5 per group. *P<.05 versus WKY group; yP<.05 versus Ad-null SHR group. WKY, Wistar-Kyoto rat; SHR, spontaneously hypertensive rat; Ad-null, control adenovirus; Ad-BRCA1, BRCA1 adenovirus.
    Figure Legend Snippet: FIGURE 8. Aortas from Ad-BRCA1–treated SHR rats exhibit more RAD51 foci formation than those from Ad-null–treated SHR rats. RAD51 foci were quantified via immunohistochemistry. Images are representative of n ¼ 5 per group. *P<.05 versus WKY group; yP<.05 versus Ad-null SHR group. WKY, Wistar-Kyoto rat; SHR, spontaneously hypertensive rat; Ad-null, control adenovirus; Ad-BRCA1, BRCA1 adenovirus.

    Techniques Used: Immunohistochemistry, Control



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    custom designed brca1 adenovirus  (Vector Biolabs)
    96
    Vector Biolabs custom designed brca1 adenovirus
    FIGURE 1. <t>BRCA1</t> is constitutively expressed in aortic SMCs. A, Representative agarose gel image and (B) immunoblot confirming the constitutive presenceofBRCA1transcriptandproteininC57Bl/6JMASMCs, WKY RASMCs and HASMCs. n ¼ 5 per group. SMC, Smooth muscle cells; BRCA1, breast cancer 1, early onset gene; MW, molecular weight marker; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.
    Custom Designed Brca1 Adenovirus, supplied by Vector Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/custom designed brca1 adenovirus/product/Vector Biolabs
    Average 96 stars, based on 1 article reviews
    custom designed brca1 adenovirus - by Bioz Stars, 2026-05
    96/100 stars
    		  Buy from Supplier

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    FIGURE 1. BRCA1 is constitutively expressed in aortic SMCs. A, Representative agarose gel image and (B) immunoblot confirming the constitutive presenceofBRCA1transcriptandproteininC57Bl/6JMASMCs, WKY RASMCs and HASMCs. n ¼ 5 per group. SMC, Smooth muscle cells; BRCA1, breast cancer 1, early onset gene; MW, molecular weight marker; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.

    Journal: The Journal of thoracic and cardiovascular surgery

    Article Title: BRCA1 shields vascular smooth muscle cells from oxidative stress.

    doi: 10.1016/j.jtcvs.2013.09.060

    Figure Lengend Snippet: FIGURE 1. BRCA1 is constitutively expressed in aortic SMCs. A, Representative agarose gel image and (B) immunoblot confirming the constitutive presenceofBRCA1transcriptandproteininC57Bl/6JMASMCs, WKY RASMCs and HASMCs. n ¼ 5 per group. SMC, Smooth muscle cells; BRCA1, breast cancer 1, early onset gene; MW, molecular weight marker; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.

    Article Snippet: The custom-designed BRCA1 adenovirus (Ad-BRCA1; containing the complete coding sequence of human BRCA1 [accession no. NM_007294.2]) and the control adenovirus Ad-null were from Vector BioLabs (Philadelphia, Penn).

    Techniques: Agarose Gel Electrophoresis, Western Blot, Molecular Weight, Marker

    FIGURE 2. BRCA1 overexpression reduces oxidative stress in HASMCs via the NADPH oxidase system. ROS production was determined by (A) CM-DCF and (B) DHE fluorescence in HASMCs stressed for 24 hours with H2O2. Representative Western blots and semiquantitative data for the NADPH oxidase subunits (C) Nox1 and (D). p47phox in naive and adenovirus-treated HASMCs. GAPDH acted as the housekeeping protein. n ¼ 5 per group. *P <.05 versus the corresponding untreated group; yP <.05 versus the H2O2-treated Ad-null–transfected group. CM-DCF, 6-Chloromethyl- 20,70-dichlorodihydrofluorescein diacetate, acetyl ester; Ad-null, control adenovirus; Ad-BRCA1, BRCA1 adenovirus; DHE, dihydroethidium; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.

    Journal: The Journal of thoracic and cardiovascular surgery

    Article Title: BRCA1 shields vascular smooth muscle cells from oxidative stress.

    doi: 10.1016/j.jtcvs.2013.09.060

    Figure Lengend Snippet: FIGURE 2. BRCA1 overexpression reduces oxidative stress in HASMCs via the NADPH oxidase system. ROS production was determined by (A) CM-DCF and (B) DHE fluorescence in HASMCs stressed for 24 hours with H2O2. Representative Western blots and semiquantitative data for the NADPH oxidase subunits (C) Nox1 and (D). p47phox in naive and adenovirus-treated HASMCs. GAPDH acted as the housekeeping protein. n ¼ 5 per group. *P <.05 versus the corresponding untreated group; yP <.05 versus the H2O2-treated Ad-null–transfected group. CM-DCF, 6-Chloromethyl- 20,70-dichlorodihydrofluorescein diacetate, acetyl ester; Ad-null, control adenovirus; Ad-BRCA1, BRCA1 adenovirus; DHE, dihydroethidium; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.

    Article Snippet: The custom-designed BRCA1 adenovirus (Ad-BRCA1; containing the complete coding sequence of human BRCA1 [accession no. NM_007294.2]) and the control adenovirus Ad-null were from Vector BioLabs (Philadelphia, Penn).

    Techniques: Over Expression, Western Blot, Transfection, Control

    FIGURE 3. Aortas and RASMCs from SHR rats express less BRCA1 than those from WKY rats. BRCA1 transcripts in (A) WKY and SHR rat aorta and (B) RASMC homogenates were assessed via qPCR. n ¼ 5 per group. *P<.05 versus WKY group. BRCA1, Breast cancer 1, early onset gene; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; WKY, Wistar- Kyoto rat; SHR, spontaneously hypertensive rat; RASMC, rat aortic smooth muscle cells.

    Journal: The Journal of thoracic and cardiovascular surgery

    Article Title: BRCA1 shields vascular smooth muscle cells from oxidative stress.

    doi: 10.1016/j.jtcvs.2013.09.060

    Figure Lengend Snippet: FIGURE 3. Aortas and RASMCs from SHR rats express less BRCA1 than those from WKY rats. BRCA1 transcripts in (A) WKY and SHR rat aorta and (B) RASMC homogenates were assessed via qPCR. n ¼ 5 per group. *P<.05 versus WKY group. BRCA1, Breast cancer 1, early onset gene; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; WKY, Wistar- Kyoto rat; SHR, spontaneously hypertensive rat; RASMC, rat aortic smooth muscle cells.

    Article Snippet: The custom-designed BRCA1 adenovirus (Ad-BRCA1; containing the complete coding sequence of human BRCA1 [accession no. NM_007294.2]) and the control adenovirus Ad-null were from Vector BioLabs (Philadelphia, Penn).

    Techniques:

    FIGURE 4. BRCA1 overexpression reduces oxidative stress in SHR RASMCs via the NADPH oxidase system. A, ROS production and (B) NADPH oxidase activity were assessed by CM-DCF fluorescence and lucigenin-enhanced chemiluminescence, respectively, in homogenates of WKY RASMCs and adenovirus- transfectedSHRRASMCs(n¼ 6pergroup).Representative WesternblotsandsemiquantitativedatafortheNADPHoxidasesubunits(C)Nox1and(D)p47phox in WKYand SHR RASMC homogenates. n ¼ 5 per group. *P<.05 versus WKY group; yP<.05 versus Ad-null SHR group. CM-DCF, 6-Chloromethyl-20,70- dichlorodihydrofluorescein diacetate, acetyl ester; WKY, Wistar-Kyoto rat; Ad-null, control adenovirus; Ad-BRCA1, BRCA1 adenovirus; SHR, spontaneously hypertensive rat; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; NADPH, nicotinamide adenine dinucleotide phosphate.

    Journal: The Journal of thoracic and cardiovascular surgery

    Article Title: BRCA1 shields vascular smooth muscle cells from oxidative stress.

    doi: 10.1016/j.jtcvs.2013.09.060

    Figure Lengend Snippet: FIGURE 4. BRCA1 overexpression reduces oxidative stress in SHR RASMCs via the NADPH oxidase system. A, ROS production and (B) NADPH oxidase activity were assessed by CM-DCF fluorescence and lucigenin-enhanced chemiluminescence, respectively, in homogenates of WKY RASMCs and adenovirus- transfectedSHRRASMCs(n¼ 6pergroup).Representative WesternblotsandsemiquantitativedatafortheNADPHoxidasesubunits(C)Nox1and(D)p47phox in WKYand SHR RASMC homogenates. n ¼ 5 per group. *P<.05 versus WKY group; yP<.05 versus Ad-null SHR group. CM-DCF, 6-Chloromethyl-20,70- dichlorodihydrofluorescein diacetate, acetyl ester; WKY, Wistar-Kyoto rat; Ad-null, control adenovirus; Ad-BRCA1, BRCA1 adenovirus; SHR, spontaneously hypertensive rat; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; NADPH, nicotinamide adenine dinucleotide phosphate.

    Article Snippet: The custom-designed BRCA1 adenovirus (Ad-BRCA1; containing the complete coding sequence of human BRCA1 [accession no. NM_007294.2]) and the control adenovirus Ad-null were from Vector BioLabs (Philadelphia, Penn).

    Techniques: Over Expression, Activity Assay, Control

    FIGURE 5. BRCA1 overexpression lowers systolic and diastolic blood pressure in SHR rats. A, Blood pressure and (B) heart rates were continuously recorded via radiotelemetry in SHR rats treated with either Ad-null or Ad-BRCA1 (5 3 1010 IU/rat, intravenously). n ¼ 9 per group. *P < .05 versus Ad-null. Ad-null, Control adenovirus; Ad-BRCA1, BRCA1 adenovirus.

    Journal: The Journal of thoracic and cardiovascular surgery

    Article Title: BRCA1 shields vascular smooth muscle cells from oxidative stress.

    doi: 10.1016/j.jtcvs.2013.09.060

    Figure Lengend Snippet: FIGURE 5. BRCA1 overexpression lowers systolic and diastolic blood pressure in SHR rats. A, Blood pressure and (B) heart rates were continuously recorded via radiotelemetry in SHR rats treated with either Ad-null or Ad-BRCA1 (5 3 1010 IU/rat, intravenously). n ¼ 9 per group. *P < .05 versus Ad-null. Ad-null, Control adenovirus; Ad-BRCA1, BRCA1 adenovirus.

    Article Snippet: The custom-designed BRCA1 adenovirus (Ad-BRCA1; containing the complete coding sequence of human BRCA1 [accession no. NM_007294.2]) and the control adenovirus Ad-null were from Vector BioLabs (Philadelphia, Penn).

    Techniques: Over Expression, Control

    FIGURE 6. BRCA1 overexpression reduces aortic oxidative stress in SHR rats via the NADPH oxidase system. A, ROS production (n ¼ 10 per group) and (B) NADPH oxidase activity (n ¼ 6 pergroup) in aorta homogenatesfrom WKYand adenovirus-treated SHR rats were assessed by lucigenin-enhanced chem- iluminescence. C, Representative Western blots and semiquantitative data for the NADPH oxidase subunits Nox1 and p47phox in WKY and adenovirus- treated SHR rat aortas. GAPDH acted as the housekeeping protein. n ¼ 6 per group. *P<.05 versus WKY group; yP<.05 versus Ad-null SHR group. ROS, Reactive oxygen species; WKY, Wistar-Kyoto rat; Ad-null, control adenovirus; Ad-BRCA1, BRCA1 adenovirus; SHR, spontaneously hypertensive rat; RLU, relative light units; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; NADPH, nicotinamide adenine dinucleotide phosphate.

    Journal: The Journal of thoracic and cardiovascular surgery

    Article Title: BRCA1 shields vascular smooth muscle cells from oxidative stress.

    doi: 10.1016/j.jtcvs.2013.09.060

    Figure Lengend Snippet: FIGURE 6. BRCA1 overexpression reduces aortic oxidative stress in SHR rats via the NADPH oxidase system. A, ROS production (n ¼ 10 per group) and (B) NADPH oxidase activity (n ¼ 6 pergroup) in aorta homogenatesfrom WKYand adenovirus-treated SHR rats were assessed by lucigenin-enhanced chem- iluminescence. C, Representative Western blots and semiquantitative data for the NADPH oxidase subunits Nox1 and p47phox in WKY and adenovirus- treated SHR rat aortas. GAPDH acted as the housekeeping protein. n ¼ 6 per group. *P<.05 versus WKY group; yP<.05 versus Ad-null SHR group. ROS, Reactive oxygen species; WKY, Wistar-Kyoto rat; Ad-null, control adenovirus; Ad-BRCA1, BRCA1 adenovirus; SHR, spontaneously hypertensive rat; RLU, relative light units; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; NADPH, nicotinamide adenine dinucleotide phosphate.

    Article Snippet: The custom-designed BRCA1 adenovirus (Ad-BRCA1; containing the complete coding sequence of human BRCA1 [accession no. NM_007294.2]) and the control adenovirus Ad-null were from Vector BioLabs (Philadelphia, Penn).

    Techniques: Over Expression, Activity Assay, Western Blot, Control

    FIGURE 7. Aortas from Ad-BRCA1–treated SHR rats exhibit less H2A.X phosphorylation than those from Ad-null–treated SHR rats. gH2A.X levels were assessed via immunohistochemistry. Images are representative of n ¼ 5 per group. *P <.05 versus WKY group; yP <.05 versus Ad-null SHR group. WKY, Wistar-Kyoto rat; SHR, spontaneously hypertensive rat; Ad-null, control adenovirus; Ad-BRCA1, BRCA1 adenovirus.

    Journal: The Journal of thoracic and cardiovascular surgery

    Article Title: BRCA1 shields vascular smooth muscle cells from oxidative stress.

    doi: 10.1016/j.jtcvs.2013.09.060

    Figure Lengend Snippet: FIGURE 7. Aortas from Ad-BRCA1–treated SHR rats exhibit less H2A.X phosphorylation than those from Ad-null–treated SHR rats. gH2A.X levels were assessed via immunohistochemistry. Images are representative of n ¼ 5 per group. *P <.05 versus WKY group; yP <.05 versus Ad-null SHR group. WKY, Wistar-Kyoto rat; SHR, spontaneously hypertensive rat; Ad-null, control adenovirus; Ad-BRCA1, BRCA1 adenovirus.

    Article Snippet: The custom-designed BRCA1 adenovirus (Ad-BRCA1; containing the complete coding sequence of human BRCA1 [accession no. NM_007294.2]) and the control adenovirus Ad-null were from Vector BioLabs (Philadelphia, Penn).

    Techniques: Phospho-proteomics, Immunohistochemistry, Control

    FIGURE 8. Aortas from Ad-BRCA1–treated SHR rats exhibit more RAD51 foci formation than those from Ad-null–treated SHR rats. RAD51 foci were quantified via immunohistochemistry. Images are representative of n ¼ 5 per group. *P<.05 versus WKY group; yP<.05 versus Ad-null SHR group. WKY, Wistar-Kyoto rat; SHR, spontaneously hypertensive rat; Ad-null, control adenovirus; Ad-BRCA1, BRCA1 adenovirus.

    Journal: The Journal of thoracic and cardiovascular surgery

    Article Title: BRCA1 shields vascular smooth muscle cells from oxidative stress.

    doi: 10.1016/j.jtcvs.2013.09.060

    Figure Lengend Snippet: FIGURE 8. Aortas from Ad-BRCA1–treated SHR rats exhibit more RAD51 foci formation than those from Ad-null–treated SHR rats. RAD51 foci were quantified via immunohistochemistry. Images are representative of n ¼ 5 per group. *P<.05 versus WKY group; yP<.05 versus Ad-null SHR group. WKY, Wistar-Kyoto rat; SHR, spontaneously hypertensive rat; Ad-null, control adenovirus; Ad-BRCA1, BRCA1 adenovirus.

    Article Snippet: The custom-designed BRCA1 adenovirus (Ad-BRCA1; containing the complete coding sequence of human BRCA1 [accession no. NM_007294.2]) and the control adenovirus Ad-null were from Vector BioLabs (Philadelphia, Penn).

    Techniques: Immunohistochemistry, Control